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This product contains the Luna Universal qPCR Master Mix, provided in volumes sufficient for preparation of up to 200 reactions (NEB #M3003S), 500 reactions (NEB #M3003L) or 1,000 reactions (NEB #M3003X) in 1 ml aliquots, or 2,500 reactions (NEB #M3004E) in a 25 ml bottle. Required Materials Not Included Target-specific primers Nuclease-free water
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1 INSTRUCTION MANUAL NEBuilder® HiFi DNA Assembly Master Mix/ NEBuilder HiFi DNA Assembly Cloning Kit NEB #E2621S/L/X, #E5520S 10/50/250 reactions Version 3.1_9/21 Table of Contents
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Product Information. Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. (1-4). M-MuLV Reverse Transcriptase lacks 3´ → 5´ exonuclease activity.
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It's difficult to reach all of Nebraska without the ability to drive, and getting a driver's license starts with a driver's permit, and the process to do that starts right here with studying the Nebraska Driver's Handbook. On this page, you'll find only the very latest version, which we pull directly from the Nebraska Department of Motor Vehicles, so you never have to worry about having ...
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240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected]
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Table of Contents. 314. A company must, in the exercise of the powers granted by this Act or a Special Act, do as little damage as possible, and must make full compensation in the manner provided in this Act and in a Special Act, to all persons interested, for all damage sustained by them by reason of the exercise of those powers. 320.
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ISO New England issues manuals outlining the procedures for various market participant responsibilities related to the region’s wholesale electricity markets and power system, consistent with associated ISO Tariff provisions.. To access previous versions of these manuals, visit the Archived Manuals page.
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Model D11 R&M Spacemaster I - 25 to 30 ton Basemounted- Maintenance Instructions and Spare Parts. PDF. Model D11 R&M Spacemaster I - 25 to 30 ton Double Girder- Maintenance Instructions and Spare Parts. PDF. Model D11 R&M Spacemaster I - 7.5 to 10 ton Monorail - Maintenance Instructions and Spare Parts. PDF.
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M.CviPI is incubated with 1 µg λ DNA in 20 µl 1X GC Reaction Buffer and 160 µM S-adenosylmethionine, for one hour at 37°C. The extent of protection by M.CviPI is determined by the addition of 30 µl NEBuffer 2 containing 10 units of HaeIII restriction endonuclease. Incubation for 1 hour at 37°C is followed by analysis on an agarose gel.
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Jan 26, 2015 . Refer to the manual (NEB #E7335) for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers. Refer to the manual for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers.
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Product Information. ProtoScript® II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of ...
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240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected]
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Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.
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To make sure that your I-neb works properly and lasts a long time, follow the important instructions in this user guide. The I-neb is a ‘clean, non-sterile’ product and is for single patient use only. The I-neb can only be used with the battery and charger supplied.
The I-neb is a ‘clean, non-sterile’ product and is for single patient use only. The I-neb can only be used with the battery and charger supplied. Do not attempt to take the I-neb body apart or tamper with the software or hardware in this product. Tested to IEC60601-1 and IEC60601-1-2.
• The I-neb has been developed for adults and children aged 2 and upwards who can co-ordinate breathing into a mouthpiece. The I-neb should always be used in conjunction with patient data supplied by the specific drug licence. • I-neb should only be used on conscious and spontaneously breathing patients.
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start TaqDNA Polymerase and has been
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