Nano Temper

Listing Results Nano Temper

About 19 results and 7 answers.

Manual Loading - NanoTemper Technologies

Prometheus NT.Plex - manual loading. The capillary chips can easily be filled by capillary action with your sample prepared in 384-well plates without any further manual pipetting or loading steps. The Prometheus NT.Plex instrument is based on nanoDSF technology for easy, rapid and accurate analysis of protein folding and stability.

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NanoTemper - Tools for Challenging Biophysical

High-quality biophysical characterization tools to help researchers study challenging molecular interactions, protein stability, and protein characterization.

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High-Resolution Protein Stability ... - NanoTemper

Proteins with a higher T m are more stable because a greater input of energy is required to reach the unfolding transition. Because Tm is an accurate and established metric for assessing protein stability, it’s an essential parameter to determine. T onset. The detectable temperature at which a …

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Systems to measure binding affinities using ...

Our Monolith systems use MicroScale Thermophoresis technology to easily measure binding affinities for a broad range of molecules, in solution using very little sample.

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nanoDSF • Protein Stability and Unfolding • Buffer and

NanoDSF stands for the nano-format of Differential Scanning Fluorimetry (DSF). It is a fast, robust, high-quality, and – most importantly – label-free and in-solution method for the analysis of protein stability, thermal protein unfolding and melting temperature analysis. Consequently, nanoDSF is a great tool for buffer and formulation ...

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Monolith Series Consumables - NanoTemper

The kit is optimized for proteins with a molecular weight higher than 5 kDa and concentrations between 2 and 20 µM. RED dyes are suited for Monolith NT.115 series and Monolith NT.Automated instruments with a RED detector (Nano and Pico) and Dianthus NT.23 series. Contents require storage at 4 °C and …

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Tycho for Protein Purification Optimization ... - NanoTemper

Tycho verifies protein quality by looking at the structural integrity (or foldedness) of a protein. As a thermal ramp is applied, the changes in the intrinsic fluorescence, detected at both 350 nm and 330 nm, from tryptophan and tyrosine residues in the protein are measured.

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Learn about Prometheus Panta - NanoTemper

Prometheus Panta reports a thorough profile of your candidate molecule’s stability with a complete set of parameters for size analysis, thermal unfolding, and aggregation. To do this it uses DLS, nanoDSF, and backreflection technologies to give you a full picture of your candidate’s stability. View Specifications.

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nanoDSF as screening tool for enzyme libraries and

The Prometheus nanoDSF instrument (NanoTemper Technologies, Munich, Germany) can measure 48 sam-ples in parallel with a capillary loading volume of only 10 lL, thereby consuming < 10 lg of protein per determination. This technique was recently tested to determine T m of two different overexpressed proteins in crude extracts.

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The Anova Precision® Cooker Quick Start Guide – Anova

Dec 30, 2016 . The manual writers haven’t a clue, Comment actions Permalink. Linda Halpin January 10, 2019 14:42. Useless manual! The online manual is no different than the printed one included with the device. I am about ready to donate this cooking tool to someone who has the …

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Protein labeling - NanoTemper Technologies

MicroScale Thermophoresis (MST) is a biophysical technique that measures the strength of the interaction between two molecules by detecting variations in fluorescence signal as a result of an IR-laser induced temperature change.

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Frequently Asked Questions

  • What can you do with a nanotemper tool?

    TARGETS Membrane Proteins Characterize in solution with any buffer or detergent RESEARCH AREAS Plant Science Improve your plant science research Virology Unravel virus biology complexity NANOTEMPER About Get to know us

  • How are nanotemper tools used to characterize proteins?

    NanoTemper tools are the quickest way to characterize proteins. with just a tiny amount of sample. Screen for hits Measure binding affinity Characterize protein stability Identify protein quality. See why Prometheus is the new gold standard that precisely characterizes protein stability.

  • How is the nanotemper used in Prometheus consumables?

    With over 10 years of experience, we’re proud that our patented capillary technology minimizes undesirable effects to proteins, while delivering the quality and consistency associated with NanoTemper Technologies. Need a quote for Prometheus consumables?

  • What can be done with a monolith nanotemper kit?

    The kit is used for site-specific and purification-free labeling of His-tagged (6xHis) proteins and peptides. The kit can be used with purified as well as crude samples (e.g. cell lysate) and contains material sufficient for 3840 single-point MST measurements.

  • When does monolith mo.control start to run?

    Most software starts once you load your samples and start your run. Monolith’s MO.Control is built differently — not only do you get help with guided step-by-step experimental planning and assay setup before you start your run, but you also get immediate feedback on assay optimization based on your results after the run is over.

  • How is monolith used to measure binding events?

    Monolith uses MicroScale Thermophoresis (MST) technology to quantify binding events. One partner is typically labeled with a fluorescent dye and becomes an extremely sensitive reporter for all molecular interactions. These events, weak or strong, cause a change in MST signal that is easily detected.

  • Is there a label free option for Monolith Pico?

    *Monolith Pico and Monolith come with a label-free option to characterize interactions under native conditions. Read the technical note to learn why having the flexibility to use either label-free or labeled target MST can benefit your research.

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