8 Tips For Using The NanoDrop To Measure DNA/RNA
Always clean the pedestal before use. In our lab, it is best practice to clean the NanoDrop’s pedestal before and...
Always measure your blank. After blanking the NanoDrop, I find it useful to then measure the blank to see if the...
Check for air bubbles. Since the NanoDrop can use volumes as low as 1 μL to...
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8 Tips For Using The NanoDrop To Measure DNA/RNA 1. Always clean the pedestal before use. In our lab, it is best practice to clean the NanoDrop’s …
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NanoDrop Products Guide. Pipette. Measure. Know. Quantify DNA, RNA and protein samples in seconds with only 1–2 µL using Thermo Scientific NanoDrop instruments. Whether you choose a NanoDrop UV-Vis spectrophotometer or fluorospectrometer, you can rely on patented* NanoDrop technology for accurate, fast results to assess sample concentration ...
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Use Of The NanoDrop Spectrophotometer. Open the software of the NanoDrop by double clicking at the icon “ND-1000 V.3.2.1” on the desktop. To measure the protein concentration at 280 nm press the “Protein A280” button. The programme will ask to place 2 l of deionized water on the lower sample pedestal and then press OK.
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Quickly and easily quantify samples. The technological foundation of every Thermo Scientific NanoDrop product is based upon the unqiue sample-retention system. This system allows scientists to quickly and easily quantify and assess purity of samples such as proteins and nucleic acids. Relevant for virtually every lab that deals with biological samples, NanoDrop …
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NanoDrop Products Guide ... Quantify DNA, RNA and protein samples in seconds with only 1–2 µL using Thermo Scientific NanoDrop instruments. Whether you choose a NanoDrop UV-Vis spectrophotometer or fluorospectrometer, you can rely on patented* NanoDrop technology for accurate, fast results to assess sample concentration and purity. ...
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NanoDrop Products Guide. Pipette. Measure. Know. Quantify DNA, RNA and protein samples in seconds with only 1–2 µL using Thermo Scientific NanoDrop instruments. Whether you choose a NanoDrop UV-Vis spectrophotometer or fluorospectrometer, you can rely on patented* NanoDrop technology for accurate, fast results to assess sample concentration ...
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NanoDrop products use a unique sample retention technology that allow UV-Vis measurements to be made from 1µL of sample with no cuvettes or dilutions. The NanoDrop family of products began with the popular NanoDrop 1000 Spectrophotometer, which made absorbance measurements fast and easy.
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How do I find out my Alberta Health Care number? Call 780-427-1432 (Dial 310-0000 first for toll free calling within Alberta)
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And speaking of NanoDrop, what is the correct care for using the NanoDrop? usually i would squeeze a bit of water to clean the probe, then very gently wipe the surface dry with a kimwipe. i'd do this before blanking and after i measure the last sample. i learnt this from my previous mentor from a previous lab and have been doing this ever since.
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Check for air bubbles Since the NanoDrop can use volumes as low as 1 μL to measure the absorbance of the sample, any tiny air bubbles present in the sample will wreak havoc on your results. Take care when pipetting and ensure all of the sample is deposited onto the centre (the black dot) of the bottom pedestal.
The primary maintenance requirement of the NanoDrop 1000 Spectrophotometer is keeping the measurement pedestal surfaces clean. Upon completion of each sample measurement, wipe the sample from the upper and lower pedestals to prevent sample carryover and avoid residue buildup.
The primary maintenance for the NanoDrop Lite is to keep the pedestal surfaces clean. 1. Pipette 3 μl of deionized water (dH 2O) onto the bottom pedestal. Do not use a squirt bottle to apply dH 2O or any other liquid to the surface of the instrument. 2. Lower the arm to form a liquid column; let it sit for approximately 2-3 minutes. 3.
Thermo Fisher Scientific 3411 Silverside Road Bancroft Building, Suite 100 Wilmington, DE 19810 U.S.A. Telephone: 302-479-7707 Fax: 302-792-7155 E-mail: info@NanoDrop.com www.NanoDrop.com For International Support, please contact your local distributor.
By using just 1 μL of your sample, this nifty little machine will tell you the concentration and purity of your nucleic acids. All of this without paying for additional reagents! Here are some tips when using the NanoDrop to measure nucleic acids in your sample: 1. Always clean the pedestal before use
The reported limit of detection for a NanoDrop 2000 is 2 ng/μL, but I would be careful when analysing samples below 10 ng/μL. Anything lower will require a more sensitive technique, such as qPCR or the Qubit. Or maybe repeat the DNA/RNA extraction and elute in a lower volume to increase the concentration of the sample. 6.
Check for air bubbles Since the NanoDrop can use volumes as low as 1 μL to measure the absorbance of the sample, any tiny air bubbles present in the sample will wreak havoc on your results. Take care when pipetting and ensure all of the sample is deposited onto the centre (the black dot) of the bottom pedestal.
Despite the NanoDrop being an absolute godsend in the lab, it does have a small number of limitations, especially regarding sensitivity. The reported limit of detection for a NanoDrop 2000 is 2 ng/μL, but I would be careful when analysing samples below 10 ng/μL. Anything lower will require a more sensitive technique, such as qPCR or the Qubit.