What can you do with the nepa21?

And if you need to study tissue outside of the organism, the NEPA21 is also amenable to ex vivo transfer of nucleic acids to a variety of tissue types such as brain , cochlear tissue, or organoids. Nepa Gene has developed tools and techniques for transfecting embryonic tissues–or even whole embryos !–in mice, rats, and chickens.

Where can I find the genome editing article including nepa21 protocols?

The genome editing article including the NEPA21 protocols is posted on Decoded Online, Integrated DNA Technologies, Inc. See the following new publications. - Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.

What type of electrode is used in the NEPA 21?

The NEPA 21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture. The first type, inexpensive electroporation cuvettes, can be used on suspension cells without the need for expensive buffering conditions with unknown additives.

What are the specs of the nepa21 electro-kinetic transfection system?

www.bulldog-bio.com Phone: 603-570-4248 info@bulldog-bio.com NEPA21 Electro-Kinetic Transfection System SPECIFICATIONS Size 346(W) x 330(D) x113(H) mm Weight 7.5 kg Warranty 2 Year Types of pulses Poring & Transfer # pulsing steps Up to 4 # poring pulses 0-9

Nepagene Design Guide - Finder Manual

Listing Results Nepagene Design Guide

About 19 results and 4 answers.

NEPA GENE

Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.

CRISPR-Cas9 effectors facilitate generation of single-sex

Although good experimental design should, where ... autosome-encoded Cas9 with a Y-linked single-guide RNA ... through droplets of opti-MEM prior to electroporation with Nepagene 21 (5 mm). sgRNAs ...

Accessories for Electroporation Systems - nepagene.jp

Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.

Bulldog-Bio

Apr 26, 2018 . In utero, ex utero, and in ovo transfections for developmental studies Nepa Gene has developed tools and techniques for transfecting embryonic tissues–or even whole embryos!–in mice, rats, and chickens.Whether it’s specific regions in a mouse embryonic brain (click for a movie), chicken cortex, or chicken gastro regions, we can provide a protocol and an …
# of poring pulses: 0-9
Pulse details: Square-wave, direct current (DC)
# pulsing phases: Up to 4
Types of pulses: Poring and Transfer

Electroporation of human pluripotent or embryonic stem

you may use our proprietary algorithms to design custom crRNAs. If you have crRNA protospacer designs of your own or from publications, use our design checker tool to assess their on- and off-targeting potential before ordering crRNAs that are synthesized using …

Start genome editing with CRISPR-Cas9 IDT

Point, click, edit. Guaranteed.* The Alt-R™ CRISPR-Cas9 System includes all of the reagents needed for successful genome editing in your research applications based on the natural S. pyogenes CRISPR-Cas9 system.. Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide …

Stormwater Best Management Design Guide Volume 3 Basin

EPA/600/R-04/121B September 2004 Stormwater Best Management Practice Design Guide Volume 3 Basin Best Management Practices By Michael L. Clar Ecosite, Inc. Ellicott City, MD, 21042 Billy J. Barfield, P.E., Ph.D. Professor Emiritus Department of Biosystems and Agricultural Engineering Oklahoma State University Stillwater, OK 74074 Thomas P. O'Connor Urban …

CRISPR-Cas9 effectors facilitate generation of single-sex

Although good experimental design should, where possible, consider both sexes 3, 4, ... by combining an autosome-encoded Cas9 with a Y-linked single-guide RNA (sgRNA) ... Zygotes were washed through droplets of opti-MEM prior to electroporation with Nepagene 21 (5 mm). sgRNAs were prepared to 12 μM final concentration and brought to room ...

Stormwater Best Management Practice Design Guide Volume 2

EPA/600/R-04/121A September 2004 Stormwater Best Management Practice Design Guide Volume 2 Vegetative Biofilters By Michael L. Clar Exocite, Inc. Ellicott City, MD21042 Billy J. Barfield Department of Biosystems and Agricultural Engineering Oklahoma State University Stillwater, OK 74074 Thomas P. O'Connor Water Supply and Water Resources Division …

Electroporation: An alternative to microinjection for

Dec 22, 2017 . Electroporation: An alternative to microinjection for creating genetically modified rodents. The TAKE electroporation method outperforms microinjection for delivery of CRISPR genome editing reagents into rodent embryos. Obtain both mouse and iPS protocols as a starting point for your genome engineering experiments. Share on Facebook.

Folate receptor α increases chemotherapy resistance

Guide RNAs: Sense (5′‐3′) Antisense (5′‐3′) FOLR1‐1: ... (Nepagene, Chiba, Japan) with a plasmid cording the piggyBac transposase (PBase). The transfected cells were cultured in 10% FCS (Foetal Calf Serum)‐DMEM supplemented with 10 ng/ml puromycin for 10 days. ... The design of guide RNA. B, Genomic PCR for genotyping of ...

Web Page Design 10,000+ Best Web Page Designs 2022

Negative space (space free of any elements) drives the attention towards visual elements overbalancing the rest of the visual design through visual force concentrated on them. There is some difference between user interface design and …

i‐GONAD: A method for generating genome‐edited animals

Jun 14, 2019 . During the development of i-GONAD, we used newer electroporators sold by BEX (CUY21EditII) and Nepagene (NEPA21) and optimized the conditions for EP (Ohtsuka et al., 2018). Both electroporators worked well in i -GONAD experiments, indicating that i -GONAD is reproducible and robust even when different electroporators are used.

Loss-of-function mutations in the co-chaperone protein

Jan 19, 2022 . The design of gRNA and ssODN is described in fig. S5. The embryos and medium were electroporated with a 5-mm-gap electrode (CUY505P5, Nepa Gene) using the NEPA21 Super Electroporator (Nepa Gene). Mouse embryos that developed to the two-cell stage after the introduction of Cas9, gRNA, and ssODN were transferred into the oviducts of female surrogates.

Frequently Asked Questions

What can you do with the nepa21?

And if you need to study tissue outside of the organism, the NEPA21 is also amenable to ex vivo transfer of nucleic acids to a variety of tissue types such as brain , cochlear tissue, or organoids. Nepa Gene has developed tools and techniques for transfecting embryonic tissues–or even whole embryos !–in mice, rats, and chickens.
Where can I find the genome editing article including nepa21 protocols?

The genome editing article including the NEPA21 protocols is posted on Decoded Online, Integrated DNA Technologies, Inc. See the following new publications. - Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.
What type of electrode is used in the NEPA 21?

The NEPA 21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture. The first type, inexpensive electroporation cuvettes, can be used on suspension cells without the need for expensive buffering conditions with unknown additives.
What are the specs of the nepa21 electro-kinetic transfection system?

www.bulldog-bio.com Phone: 603-570-4248 [email protected] NEPA21 Electro-Kinetic Transfection System SPECIFICATIONS Size 346(W) x 330(D) x113(H) mm Weight 7.5 kg Warranty 2 Year Types of pulses Poring & Transfer # pulsing steps Up to 4 # poring pulses 0-9