(Nepa Gene) Competitor N (Company L) Competitor N (Company I (L)) Characteristics: New Electroporation No Special Buffers: Electroporation and Special Buffers: Electroporation and Special Tips: Transfection Efficiency: Cell Viability: Consumables (Disposable Kits) Economy Cuvettes USD 2.00/reaction: Special Kits USD 20.00/reaction: Special Kits USD 20.00/reaction
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Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.
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Apr 26, 2018 . Applications Support for NEPA21 Description Ordering Specifications High efficiency and high viability without special buffers The most popular transfection procedures all require specialized chemicals, which are costly—as …
# of poring pulses: 0-9
Pulse details: Square-wave, direct current (DC)
# pulsing phases: Up to 4
Types of pulses: Poring and Transfer
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Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.
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NEPA21 (Nepa Gene) Device: N (Company L) Device: N (Company I) Cuvettes ONLY No Special Buffers Transfection KitsWith Special Buffers: Transfection KitsWith Special Buffers: Cost per One Sample Euro 1.80 (€90.00/50 pcs) Cost per One Sample Euro 18.60 (€186.05/10 RCT) Cost per One Sample Euro 15.00 (€375.00/25 Tips) *Euro Price.
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We measured gene transfer efficiency using the ELEPO21 in Gram-negative bacteria. Competent cells were prepared as usual from E. coli DH5α. The competent cells were mixed with pUC19 DNA, and a 20μl aliquot (containing 10^9-10^11 cells and 10 pg DNA in 10％ glycerol solution) was transferred to the 1 mm gap electrode cuvette (EC-001S, Nepa Gene).
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Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.
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The currency code for Rupees is NPR, and the currency symbol is ₨. Below, you'll find Nepalese Rupee rates and a currency converter. You can also subscribe to our currency newsletters with daily rates and analysis, read the Xe Currency Blog, or take NPR rates on the go with our Xe Currency Apps and website.
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Pagers that are restricted to numeric-only messages are available new for $30-$50. For example, USA Mobility, which supplies many healthcare and government agencies, sells a numeric-only pager [ 1] for $39. American Messaging offers a …
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More recently, protocols utilizing AC and DC electric fields have further improved the efficiency of cell fusion – in some cases, by as much as 25 to 100-fold. The ECFG21 incorporates the latest in solid state electronic design for a robust system with plenty of useful feedback.
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Authentic Indian meals in 5 minutes. Meal kits curated by local cuisine experts across India and delivered to your doorsteps across the world. Preservative-free, ready-to-eat, healthy meals from regional cuisines of India. Plans start at $4.99. Free shipping.
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Versatile Electroporator for In Vivo, In Vitro and Much More High efficiency and high viability without special buffers The most popular transfection procedures all require specialized chemicals which are costly – running as much as $8, $12 or even $16 or more per sample.
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The Neon system uses a simple 3-step transfection procedure. The transfection occurs in the Neon Pipette tip. Load mix of harvested cells and molecules to be delivered (e.g., DNA, RNA, protein) into the Neon Pipette tip. Plug the pipette into position in the Neon transfection device; select your protocol, and press Start.
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Bulldog Bio is a full-service provider of instruments, tools, and reagents to life science researchers in North America. We make sure that you’ll have a single point-of-contact who can provide everything needed to support our products.
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Product Code: CUY512-5: Application: Electrodes for Electroporation: Description: The CUY512-5 is a Stainless Steel, Electrodes for adhesive cells with 2 plates, 5mm gap. Please note that the product number for this item was changed to CUY900-13-2-5 and it can still be supplied. At this point in time, we do not have very much data for the use of the CUY900-13-2-5.
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Being that it takes the weight of an object on earth and converts it to the weight on Neptune, the formula is Weight on Neptune= (Weight on Earth/9.81m/s2) * 11.15m/s2. To find the weight on Neptune, we divide the weight on earth by the earth's force of gravity, which is 9.81m/s 2. This calculates the mass of the object.
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Typical costs: 1 Pagers that are restricted to numeric-only messages are available new for $30-$50. ... 2 Models that allow alpha-numeric messages typically cost about $60-$100, depending on model and features. ... 3 Some alpha-numeric pagers offer full QWERTY keyboards and two-way messaging capabilities. ... More items...
And if you need to study tissue outside of the organism, the NEPA21 is also amenable to ex vivo transfer of nucleic acids to a variety of tissue types such as brain , cochlear tissue, or organoids. Nepa Gene has developed tools and techniques for transfecting embryonic tissues–or even whole embryos !–in mice, rats, and chickens.
Amazon sells a Long Range Systems server paging system [ 8] with five server-pagers that costs $507. Most pagers include a holster to clip the unit to a belt, purse or backpack. A pager should offer a selection of user alerts, such as vibrate, beep or silent.
Nepa Gene Co., Ltd.3-1-6 Shioyaki, Ichikawa, Chiba, 272-0114 JAPAN e-mail: info@Nepagene.jp web: www.Nepagene.jp *The red circle indicates a whole shape of the well-bottom of a 24-well plate.
This can be applied to adherent-cell/tissue transfection. Applications with Electroporation Cuvettes The NEPA21 Electroporator makes it possible to achieve high transfection efficiency and viability without resourse to special buffers for difficult-to-transfect cells such as PRIMARY CELLS, STEM CELLS, IMMUNE CELLS, BLOOD CELLS, etc.
The NEPA 21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture. The first type, inexpensive electroporation cuvettes, can be used on suspension cells without the need for expensive buffering conditions with unknown additives.
V: Viability, TE: Transfection Efficiency 26 NEPA21 with Electroporation Cuvettes Disposable Kits / Special Buffers NEPA21 (Nepa Gene) Nucleofector (Lonza) Neon (Invitrogen)
Step 1: Poring Pulses For forming pores(small holes) in cell membrane with low damage. Step 2: Transfer Pulses For deliveringthe target molecules into cells with low damage. NEPA21 Features •High Transfection Efficiency & High Viability Without Special Buffers