500–3,000 V (100 V resolution) Pulse length. None setting or 1.0-5.0 msec (0.5 msec resolution) Number of pulses. 1 or 2. NEPA Porator exponential decay …
Dimensions: 335 (W) x 205 (D) x 85 (H) mm
Weight: 3.2 kg
Save: 99 programs
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Specifications Nepa Gene Co., Ltd. is a Japanese manufacturer who develops state-of-the-art laboratory devices such as transfection systems and cell fusion systems. Founded in 2001, it has grown to become the preferred choice for electroporation of difficult-to-transfect cell lines.
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NEPA21 Novel 4-Step Multiple Electroporation Pulse. The novel 4-step pulse with voltage decay results in higher transfection efficiency and higher viability WITHOUT special buffers. 1) Poring Pulse Mode: higher voltage, shorter duration, multiple pulses, voltage decay. This poring pulse is for forming pores (small holes) in cell membrane with minimum damage.
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The ELEPO21 can deliver up-to 3,000 Volt pulses at millisecond (not microsecond) range. Micro Pick and Place System has been launched! The MPP-300/200/100 can easily control ultrasmall volumes (picoliter range) and transfer micro targets such as single cells one by one from one place to another place observing the targets with a microscope. Super Electroporator NEPA21 …
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The aligned cells are high-efficiently fusioned by the 2-step DC pulses with voltage decay. The post-fusion AC is applied so that the fusion process begins to mature and high fusion efficeincy is achieved with low cell damage. The efficiency by the ECFG21 is 25-100 times higher than PEG.
Duration: 0-99 sec
Post-Fusion Duration: 0-99 sec
Frequency: 1 MHz
Voltage: 0-80 Vrms
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The CUY21EDIT can be used with more than 250 NEPA GENE's CUY series electrodes which make it possible to do a wide range of electroporation experiments. And custom-made electrodes are easily ordered and available for this CUY21 electroporator. It enables you to realize the experiments which you have actually desired.
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Apr 26, 2018 . Nepa Gene has created THE most sophisticated apparatus for delivering DNA and RNA to cells—and all that’s needed are the electrodes. With a combination of low voltage short-and-long pulses and reversing polarities, a huge variety of cells , tissues, and even whole organisms can be transfected with amazingly high efficiencies.
# of poring pulses: 0-9
Pulse details: Square-wave, direct current (DC)
# pulsing phases: Up to 4
Types of pulses: Poring and Transfer
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High Transfection Efficiency & Viability Without Special Buffers. Features. In-Vitro Transfection. For Primary Cells, Stem Cells and Any Cell Lines. ・ With Electroporation Cuvettes. ・ With Adherent Cell Electrodes. For Cells in Adherence. In-Vivo Transfection.
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Delivery of Self-amplifying mRNA Vaccines by Cationic Lipid Nanoparticles: The Impact of Cationic Lipid Selection. Self-amplifying RNA (SAM) represents a versatile tool that can be used to develop potent vaccines, potentially able to elicit strong antigen-specific humoral and cellular-mediated immune responses to virtually any infectious disease.
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Bulldog Bio is a full-service provider of instruments, tools, and reagents to life science researchers in North America. We make sure that you’ll have a single point-of-contact who can provide everything needed to support our products.
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EC-001S. NEPA Electroporation Cuvettes 1mm gap with a pipette. Brown. 1.0mm. 20-70µl. 50/pkg. EC-002S. NEPA Electroporation Cuvettes 2mm gap with a pipette. Green.
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The pPB‐CAG‐FOLR1‐IRES‐Puro vector was electroporated into 1 × 10 6 MKN1 using a NEPA21 electroporation system (Nepagene, Chiba, Japan) with a plasmid cording the piggyBac transposase (PBase). The transfected cells were cultured in 10% FCS (Foetal Calf Serum)‐DMEM supplemented with 10 ng/ml puromycin for 10 days.
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Precision Antibody was founded by scientists, is run by scientists, and created for scientists. Our services have been designed by our team of immunologists and have been sought after by major pharmaceutical, biotech and diagnostic companies, together with many well-known academic and government Institutions.
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30 30 Spec RFl 500 700: 268-7364: 6400 Detector Dewar Filler Funnel (3: 0014-051: ZnSe Polarizer, 25 mm Slide: 268-8083: 8300 Flowcell Slope Cavity, 10 mm Pl Z=15: 0014-053: ZnSe Polarizer, FT80: 268-8135: 3500 Black Annodized Spacer 1 mm Cells: 0014-390: 80 Grz Angle Magna 50 Series: 268-8137: 3700 Black Annodized Spacer, 5 mm Cells: 0016-004: Paper …
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www.bulldog-bio.com Phone: 603-570-4248 [email protected] NEPA21 Electro-Kinetic Transfection System SPECIFICATIONS Size 346(W) x 330(D) x113(H) mm Weight 7.5 kg Warranty 2 Year Types of pulses Poring & Transfer # pulsing steps Up to 4 # poring pulses 0-9
NEPA21 with Electroporation Cuvettes Disposable Kits / Special Buffers NEPA21 (Nepa Gene) Nucleofector (Lonza) Neon (Invitrogen) 27 Cuvettes Special Buffers Tips Special Buffers Cuvettes Only
Super Electroporator NEPA21 Novel 4-Step Multiple Electroporation Pulse Voltage Time 1 2 3 4 Step 1 : Poring Pulse Mode Step 3: Transfer Pulse Mode Step 4: Polarity Exchanged Transfer Pulse High Voltage, Short Duration, Multiple Pulses, Voltage Decay Low Voltage, Long Duration, Multiple Pulses, Voltage Decay
With a combination of low voltage short-and-long pulses and unique polarity reversal, the NEPA21 can transfect a huge variety of cells, tissues, and even organisms with amazing efficiencies. This patented pulsing technology has been shown to be both superior to and far more flexible than other techniques.
See the performance of the NEPA21 electroporator with your cells. The novel 4-step pulse with voltage decay results in higher transfection efficiency and higher viability WITHOUT special buffers. This poring pulse is for forming pores (small holes) in cell membrane with minimum damage.
The NEPA21 electroporator and the CUY series in-vivo electrodes make it possible to transfer DNA/RNA into in vivo mice/rats. pCAGGS-lacZ that expresses beta-galactosidase was transfected into muscle via Electroporation.
The ELEPO21 In Vitro High-Voltage Electroporator developed by Nepa Gene Co., Ltd. has a unique pulsing system composed of 4-step multiple pulses with decaying, and it can achieve transformation efficiency markedly higher than traditional methods that use a single-step exponential pulse wave in bacteria, yeasts, and fungi.
The Neon Transfection System offers an innovative electroporation transfection method that utilizes a proprietary biologically compatible pipette tip chamber to generate a more uniform electric field for a significant increase in transfection efficiency and cell viability.