Related Manuals for Nepagene ELEPO21. No related manuals . Summary of Contents for Nepagene ELEPO21. Page 2: Important Information The In Vitro High Voltage Electroporator ELEPO21 is an electroporation device designed to provide a convenient way to introduce materials such as foreign DNA into sample’s cells, bacteria, fungi and yeasts. ...
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NEPA21 Applications. In Vitro Transfection. into difficult-to-transfect cells such as primary cells, stem cells, etc. into any cell lines. with electroporation cuvettes. with cell-culture-plate electrodes for cells in adherence. In Vivo Transfection. into mouse/rat brain, eye, …
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We measured gene transfer efficiency using the ELEPO21 in Gram-negative bacteria. Competent cells were prepared as usual from E. coli DH5α. The competent cells were mixed with pUC19 DNA, and a 20μl aliquot (containing 10^9-10^11 cells and 10 pg DNA in 10％ glycerol solution) was transferred to the 1 mm gap electrode cuvette (EC-001S, Nepa Gene).
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NEPA21 (Nepa Gene) Device: N (Company L) Device: N (Company I) Cuvettes ONLY No Special Buffers Transfection KitsWith Special Buffers: Transfection KitsWith Special Buffers: Cost per One Sample Euro 1.80 (€90.00/50 pcs) Cost per One Sample Euro 18.60 (€186.05/10 RCT) Cost per One Sample Euro 15.00 (€375.00/25 Tips) *Euro Price.
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Nepagene ELEPO21 Instruction Manual Instruction manual (36 pages) PASCO PS-3519 Instruction Sheet Instruction sheet (5 pages) Agilent Technologies SPS 4 User Manual Operation & user’s manual …
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This can be applied to adherent-cell/tissue transfection. Applications with Electroporation Cuvettes The NEPA21 Electroporator makes it possible to achieve high transfection efficiency and viability without resourse to special buffers for difficult-to-transfect cells such as PRIMARY CELLS, STEM CELLS, IMMUNE CELLS, BLOOD CELLS, etc.
NEPA21 1 NEPA 21 Electro-Kinetic stem cells, neurons and more Transfection System Per electrode type.
Electroporators Objects Parameters NEPA21 Electroporator All Pulses Voltage, Ampere, Time & Joule* CUY21 Electroporators (Nepa Gene’s Previous Models) One Pulse Only Voltage, Ampere & Time The Other Company’s Electroporators One Pulse Only Voltage & Time Only Setting: 100V The Results will not be the same
NEPA21 electroporator and the CUY series in-utero electrodes make it possible to transfer DNA/RNA into mouse/rat embryos (cerebral cortex, hippocampus, spinal cord, and more.)
Electroporators Objects Parameters NEPA21 Electroporator All Pulses Voltage, Ampere, Time & Joule* CUY21 Electroporators (Nepa Gene’s Previous Models) One Pulse Only Voltage, Ampere & Time The Other Company’s Electroporators One Pulse Only Voltage & Time Only Setting: 100V The Results will not be the same
NEPA21 1 NEPA 21 Electro-Kinetic stem cells, neurons and more Transfection System Per electrode type.
The ELEPO21 can deliver up-to 3,000 Volt pulses at millisecond (not microsecond) range. Micro Pick and Place System has been launched! The MPP-300/200/100 can easily control ultrasmall volumes (picoliter range) and transfer micro targets such as single cells one by one from one place to another place observing the targets with a microscope.
Up to 3,000 Volt pulses at millisecond (not microsecond) range can be delivered. Remarkably higher transformation efficiency than traditional electroporation *This instrument complies with CE marking, UL standard and CSA standard.