Tdt User Manual

Listing Results Tdt User Manual

Frequently Asked Questions

• What are the specifications of TDT?

  Specifications Product Type: TDT Concentration: 20 U/µL Quantity: 500U Enzyme: Terminal Deoxynucleotidyl Transferase Compatible Buffer: Storage Buffer, 5X Reaction Buffer

• What does TDT stand for?

  Thermo Scientific Terminal Deoxynucleotidyl Transferase (TDT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA.

• How many nucleotides are required for TDT primer?

  TDT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TDT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template.

• Why can't I use RNA as a template for TDT?

  With RNA as template TDT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template. Due to the presence of CoCl 2, the TDT Reaction Buffer is incompatible with downstream applications.

• Why choose TDT fiber photometry?

  If you are considering purchasing a fiber photometry system from us, then thank you as well - TDT is the industry leader in fiber photometry systems and we have many successful and happy customers who use our products. We would enjoy nothing more than to have you join the TDT family.

• What is the purpose of fiber photometry?

  Fiber Photometry: An imaging technique used to monitor neural activity of specifically-targeted cell populations. Fiber photometry uses excitation light from implanted fiber optics to record fluorescent activity of genetically-encoded calcium indicators (GECI) in neuronal populations.

• What is the MAX Light Power output for the photometry application?

  Fiber photometry is a low light power application, so the max output does not need to be very high. Setting the output ranges to 200 mA or greater should work well for most modern setups. Common light sources – 405 nm (autofluorescence detection, isosbestic control), 465 nm (GCaMP), 560 nm (TDTomato, mCherry, RCaMP).

• What is photphotobleaching and demodulation?

  Photobleaching (Patch Cords): The process of exposing a fiber optic patch cable to high levels of light (500mA) for a long duration (~4 hours) to reduce auto fluorescent emissions from within the cable. Demodulated: The demodulated signals are your response waveforms.