Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.
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Nepa Gene Co., Ltd. Founded Date: February 2001: Founder: Yasuhiko Hayakawa: Address: 3-1-6 Shioyaki, Ichikawa-City, Chiba 272-0114 JAPAN: Phone/Fax +81 47 306 7222/+81 47 306 7333: E-mail: info AT Nepagene.jp
Company Name: Nepa Gene Co., Ltd.
Founder: Yasuhiko Hayakawa
Founded Date: February 2001
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The NEPA21 electroporator and the CUY series in-vivo electrodes make it possible to transfer DNA/RNA into in vivo mice/rats. Transfection into In Vivo Muscle Transfection into In Vivo Brain, Retina, Skin, Testis, Liver, Kidney and More See MORE. See the Electrodes. See the Publications. NEPA21 In Utero Electroporation
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In utero, ex utero, and in ovo transfections for developmental studies Nepa Gene has developed tools and techniques for transfecting embryonic tissues–or even whole embryos!–in mice, rats, and chickens.Whether it’s specific regions in a mouse embryonic brain (click for a movie), chicken cortex, or chicken gastro regions, we can provide a protocol and an electrode set.
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Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (gap) between them of 3 mm or 5 mm (Nepa Gene Co., Ltd.) . Injection of plasmid DNA into muscle
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Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.
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Can be used to transfect large plasmids (10+ kb) Works with proteins, RNA, and gene editing complexes. Proven on hundreds of cell lines and cell types. Key publications: The NEPA21 has been referenced in more than 800 publications since 2012. Most of these use 2 mm electroporation cuvettes.
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Latest News. Systems Engineering of Microphysiometry 18. January 2022 - 10:26; Heart-on-a-Chip 17. January 2022 - 14:04; PROOF-OF-PRINCIPLE FOR IMPEDANCE MEASUREMENT WITH CARBON ELECTRODES FOR IMPLEMENTATION IN A BONE-ON-A-CHIP SYSTEM 7. July 2021 - 17:33
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New: Xceltis is the official distributor for Kingfisher Biotech. Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species. Currently, they offer over 1000 reagents and kits for veterinary ...
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10,000+ Awesome Templates Of 2022. Select from over 10,000 templates from the portfolio, business, fashion, technology, education, blog, and other trending themes. All templates are fully responsive and available for free. Last Updated: Jan 14, …
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NEOPRENE RUBBER SHEETS black, 8x8-Inch by 1/16 (Pack of 4), Gaskets DIY Material, Ideal for Domestic uses where Protection is required, Isolation, Plumbing repairs, Useful for supports 4.6 out of 5 stars 137
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Mar 05, 2019 . Gübreleme ve Suda Bitki Yetiştirme. Gübreleme yada suda bitki yetiştirme sistemi ile bitkiler yapay alt tabakada büyürken, besinler rutin olarak sulama suyu ile sağlanır. Gıda seviyesi; su içeriği ve iletkenlik (EC) gözlemi ve sulama suyuna sıvı gübre enjekte edilmesi ile ayarlanır. Delta-T WET sensör bu çok önemli bilgiyi ...
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Oct 31, 2017 . These protocols and methods are a good starting point for protocol optimization for your studies, and often offer tips for all aspects of genome editing experiments, from growth conditions through genome editing detection. Table 1. Alt-R CRISPR System protocols and user methods.*. Model system. Transfection method. IDT protocols. Cultured cells.
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Dec 22, 2017 . Outside Japan, the NEPA21 electroporator can be obtained through the Nepa Gene Company distributor network ( www.Nepagene.jp/e_about02.html ). Here you can access protocols for mouse zygote electroporation and human-induced iPS …
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Dabcyl acid Data Sheet gradients of Wnt5a surround the callosum and corticospinal tract and Wnt5a repels cortical axons in explant cultures. Hence in the callosum of knockout mice lacking Ryk receptors guidance errors had been attributed to disruption of Wnt5a/Ryk-mediated axon repulsion. ... Nepagene) which delivered 20 pulses of 10-ms ...
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A parallel plate capacitor contains two dielectric slabs of thickness d 1, d 2 and dielectric constant k 1 and k 2 respectively. The area of the capacitor plates and slabs is equal to A. Considering the capacitor as combination of two capacitors in series, the equivalent capacitance C is given by: 1 C = 1 C 1 + 1 C 2.
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Cell-Specific Transfection Protocols. NOTE: Invitrogen Lipofectamine Transfection Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can ...
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Nepa Gene Co., Ltd.3-1-6 Shioyaki, Ichikawa, Chiba, 272-0114 JAPAN e-mail: info@Nepagene.jp web: www.Nepagene.jp *The red circle indicates a whole shape of the well-bottom of a 24-well plate.
And if you need to study tissue outside of the organism, the NEPA21 is also amenable to ex vivo transfer of nucleic acids to a variety of tissue types such as brain , cochlear tissue, or organoids. Nepa Gene has developed tools and techniques for transfecting embryonic tissues–or even whole embryos !–in mice, rats, and chickens.
Electro-Kinetic Transfection System www.bulldog-bio.com Phone: 603-570-4248 [email protected] Typical 4-Step Electro-kinetic Protocol In vitro transfection of suspension, adherent, and primary cells The NEPA21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture.
The NEPA 21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture. The first type, inexpensive electroporation cuvettes, can be used on suspension cells without the need for expensive buffering conditions with unknown additives.