Nepa Gene Co., Ltd. is a sicentific manufacturer that has developed state-of-art laboratory devices such as transfection systems and cell fusion systems.
Show more
See More
NEPA21 Cuvette Electroporation. It is possible to achieve high transfection efficiency and high viability without resourse to special buffers for difficult-to-transfect cells such as primary cells, stem cells, immune cells, blood cells, etc.
Show more
See More
1. At first use, resuspend the Alt-R Cas9 Electroporation Enhancer or ssODN to 100 µM in Nuclease-Free Duplex Buffer to create a stock solution. 2. For each set of experiments, dilute stock (from step D1) to 10.8 µM in Neon Resuspension Buffer R to create a working solution. Note: You will need 2 µL of working solution for each electroporation. For assistance, use the
Show more
See More
cause of its efficiency and ease of use (43). This technique employs target-recognizing short guide RNA (sgRNA) and Cas9 nuclease, which was origi-nally isolated from Streptococcus pyogenes (28). During the gene-editing process, the addition of do-nor DNA enables us to reconstitute genes of interest (22). Taking advantage of this technique, a ...
Show more
See More
This DNA repair gene functions in multiple biological processes, including meiosis. However, different meiotic studies necessitate males or females. Male Atm mutants are used to study recombination...
Show more
See More
Dec 10, 2018 . Background Oxygen-evolving photosynthetic microorganisms, collectively termed as microalgae, are gaining attention as alternative fuel sources. The unicellular alga Coccomyxa sp. strain KJ that belongs to the class Trebouxiophyceae can grow rapidly in minimal mineral media and accumulate triacylglycerols at levels > 60% (w/w) of its dry weight under nitrogen …
Show more
See More
Point, click, edit. Guaranteed.* The Alt-R™ CRISPR-Cas9 System includes all of the reagents needed for successful genome editing in your research applications based on the natural S. pyogenes CRISPR-Cas9 system.. Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide …
Show more
See More
Lipid nanoparticle–based delivery of mRNA encoding an ABE and a single-guide RNA targeting PCSK9, a negative regulator of LDL, induced up to 67% editing (on average, 61%) in mice and up to 34% ...
Show more
See More
Nepagene ELEPO21 Instruction Manual Instruction manual (36 pages) Linkam Scientific Instruments LTS420 User Manual Operation & user’s manual (28 pages) Organomation MICROVAP 11801 Instruction Manual Instruction manual (32 pages) Kontron SFM25 Instruction Manual Instruction manual (97 pages)
Show more
See More
Nahita 631/45 Plus Manuals & User Guides. User Manuals, Guides and Specifications for your Nahita 631/45 Plus Laboratory Equipment. Database contains 1 Nahita 631/45 Plus Manuals (available for free online viewing or downloading in PDF): Manual .
Show more
See More
1 (888)302-2675 1 (888)814-4206. Please note. All our papers are written from scratch. To ensure History Of Christian Names high quality of writing, the pages number is limited for short deadlines. If you want to order more pages, please choose longer Deadline (Urgency). does everything it says it will do and on time.
Show more
See More
Alternate Protocol 1: Transient CRISPR Cas9-gRNA delivery for gene knockout by Nepagene electroporator. Basic Protocol 2: FACS and single-cell clone generation. Alternate Protocol 2: Manual cell dilution to obtain single-cell clones. Basic Protocol 3: Confirming indels status in single-cell clones by PCR on genomic DNA and Sanger sequencing
Show more
See More
And if you need to study tissue outside of the organism, the NEPA21 offers specialized electrodes for the ex vivo transfer of nucleic acids to a variety of tissue explants including brain. In utero, ex uteroand in ovotransfections for developmental studies
NEPA21 with Electroporation Cuvettes Disposable Kits / Special Buffers NEPA21 (Nepa Gene) Nucleofector (Lonza) Neon (Invitrogen) 27 Cuvettes Special Buffers Tips Special Buffers Cuvettes Only
The genome editing article including the NEPA21 protocols is posted on Decoded Online, Integrated DNA Technologies, Inc. See the following new publications. - Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.
Step 1: Poring Pulses For forming pores(small holes) in cell membrane with low damage. Step 2: Transfer Pulses For deliveringthe target molecules into cells with low damage. NEPA21 Features •High Transfection Efficiency & High Viability Without Special Buffers
The NEPA21 Transfecton System’s 4-step pulse with voltage decay results in higher transfection efficiency and lower cell damage without the use of special buffers. Step 1 – Poring Pulse: High voltage, short duration, multiple pulses, voltage decay. The poring pulse is for forming small holes in the cell membrane with minimal damage.
Super Electroporator NEPA21 has been launched! The NEPA21 can achieve high transfection efficiency and high viability WITHOUT special buffer. Super Electro Cell Fusion Generator ECFG21 has been launched! The cell fusion efficiency is dramatically higher than PEG!
V: Viability, TE: Transfection Efficiency 26 NEPA21 with Electroporation Cuvettes Disposable Kits / Special Buffers NEPA21 (Nepa Gene) Nucleofector (Lonza) Neon (Invitrogen)
CUY21 Electroporators (Nepa Gene’s Previous Models) One Pulse Only Voltage, Ampere & Time The Other Company’s Electroporators One Pulse Only Voltage & Time Only Setting: 100V The Results will not be the same *Joule (energy) = V x A x T